KMID : 0366220140490040265
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Korean Journal of Hematology 2014 Volume.49 No. 4 p.265 ~ p.269
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Progress of in vitro factor VIII coagulant activity from 0 to 8 hours after reconstitution
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Shim Ye-Jee
Lee Kun-Soo Kim Uk-Hyun Suh Jin-Kyung Baik Sae-Yun Hyun Shin-Young
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Abstract
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Background: Continuous infusion of factor VIII (FVIII) is a more cost-effective method for treating hemo-philia A than intermittent bolus injection. However, there is currently no specific data in Korea about the progress of in vitro FVIII coagulant activity (FVIII:C) after reconstitution from its lyophilized form.
Methods: Three commercial FVIII concentrate products (two recombinant FVIII and one plasma-de-rived) were used. In vitro FVIII:C was measured at 0, 2, 4, 6, and 8 hours following recon-stitution in both the indoor light-exposed and light-shielded groups.
Results: For the three drugs, in vitro FVIII:C decreased over the 8 hours following reconstitution (P£¼0.001). The decline of FVIII:C was linear (P£¼0.001). In vitro FVIII:C for the indoor light-exposed groups was 95.3¡¾1.9% and 90.6¡¾2.5% after 4 and 8 hours following recon-stitution, respectively, compared to baseline activity. In the light-shielded group, FVIII:C was 95.4¡¾1.1% and 90.9¡¾1.7% of the baseline activity after 4 and 8 hours, respectively. There was no statistical difference between FVIII:C in the indoor light-exposed and light-shielded groups (P=0.849).
Conclusion: In vitro FVIII:C decreased after reconstitution, but activity was maintained at over 90% of the baseline value during 8 hours. Exposure to indoor light did not accelerate the loss of FVIII:C over the experimental time. This result indicates that CI with FVIII is available in 8-hour intervals, with no indoor light-exposure precautions needed.
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KEYWORD
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Hemophilia A, Continuous infusion, Factor VIII
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